| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 37..55 | Fwd | |
|
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
|
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| TEV site | — | tobacco etch virus (TEV) protease recog… | 79..100 | Fwd | |
|
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
|
|||||
| 6xHis | — | 6xHis affinity tag | 444..462 | Fwd | |
|
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| f1 ori | — | — | 612..971 | Fwd | |
|
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 528..576 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
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