| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| TEV site | — | tobacco etch virus (TEV) protease recog… | 410..431 | Fwd | |
|
DNA Sequence
GAGAACCTCTACTTCCAATCG
Protein Sequence
ENLYFQS
|
|||||
| 6xHis | — | 6xHis affinity tag | 431..449 | Fwd | |
|
DNA Sequence
CACCATCATCACCACCAT
Protein Sequence
HHHHHH
|
|||||
| FLAG | — | FLAG(R) epitope tag, followed by an ent… | 449..473 | Fwd | |
|
DNA Sequence
GATTACAAGGATGACGACGATAAG
Protein Sequence
DYKDDDDK
|
|||||
| 6xHis | — | 6xHis affinity tag | 522..540 | Fwd | |
|
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 606..654 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
|||||