PHF8

39032 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
PHF8 (PHF8 Human)
Tags
His6-Thioredoxin-TEV
Available Since
March 18, 2013
Availability
Available

Sequence Information

Length
914 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 145..163 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
TrxA trxA E. coli thioredoxin 172..499 Fwd
DNA Sequence
ATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGCGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCC
Protein Sequence
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
TEV site tobacco etch virus (TEV) protease recog… 505..526 Fwd
DNA Sequence
GAGAACTTGTACTTCCAATCC
Protein Sequence
ENLYFQS
promoter 1
Label Gene Product Position Strand
T7 promoter 54..73 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator 73..98 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC