HDHD3

39029 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
HDHD3 (HDHD3 Human)
Tags
TEV-His6-Flag
Available Since
March 18, 2013
Availability
Available

Sequence Information

Length
1,022 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
TEV site tobacco etch virus (TEV) protease recog… 767..788 Fwd
DNA Sequence
GAGAACCTCTACTTCCAATCG
Protein Sequence
ENLYFQS
6xHis 6xHis affinity tag 788..806 Fwd
DNA Sequence
CACCATCATCACCACCAT
Protein Sequence
HHHHHH
FLAG FLAG(R) epitope tag, followed by an ent… 806..830 Fwd
DNA Sequence
GATTACAAGGATGACGACGATAAG
Protein Sequence
DYKDDDDK
terminator 1
Label Gene Product Position Strand
T7 terminator 966..1013 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAACGGGTCTTGAGGGGTTTTTTG