MAP3K10

38962 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
MAP3K10 (MAP3K10 Human)
Tags
His Tev
Available Since
March 18, 2013
Availability
Available

Sequence Information

Length
760 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
TEV site — tobacco etch virus (TEV) protease recog… 145..166 Rev
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
6xHis — 6xHis affinity tag 190..208 Rev
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
promoter 2
Label Gene Product Position Strand
T7 promoter — — 280..299 Rev
DNA Sequence
TAATACGACTCACTATAGG
lacI promoter lacI — 607..685 Fwd
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
protein_bind 1
Label Gene Product Position Strand
lac operator — — 255..280 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC