DLG2 — PlasmidDB

Plasmid Information

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DLG2 (DLG2 Human)

Available Since

March 18, 2013

Availability

Available

Length

903 bp

Topology

linear

Molecule Type

genomic DNA

CDS 3

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	150..168	Fwd
DNA Sequence CACCATCATCATCATCAT Protein Sequence HHHHHH
TEV site	—	tobacco etch virus (TEV) protease recog…	192..213	Fwd
DNA Sequence GAGAACCTGTACTTCCAATCC Protein Sequence ENLYFQS
6xHis	—	6xHis affinity tag	581..599	Fwd
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	59..78	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	78..103	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	665..713	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG