ALDH18A1 — PlasmidDB

Plasmid Information

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ALDH18A1 (ALDH18A1 Human)

Length

852 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	142..160	Fwd
DNA Sequence CACCATCATCATCATCAT Protein Sequence HHHHHH
TEV site	—	tobacco etch virus (TEV) protease recog…	184..205	Fwd
DNA Sequence GAGAACCTGTACTTCCAATCC Protein Sequence ENLYFQS

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	51..70	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	70..95	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC