MAP3K3

38937 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
MAP3K3 (MAPK3 Human)
Tags
His Tev
Available Since
March 18, 2013
Availability
Available

Sequence Information

Length
795 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 30..48 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 380..401 Rev
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
6xHis — 6xHis affinity tag 425..443 Rev
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 515..534 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 490..515 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC