DLG3

38925 Homo sapiens Bacterial Expression

Plasmid Information

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DLG3 (DLG3 Human)

Tags

His Tev

Available Since

March 18, 2013

Availability

Available

Sequence Information

Length

972 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 3

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	16..34	Rev
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH
TEV site	—	tobacco etch virus (TEV) protease recog…	402..423	Rev
DNA Sequence GAGAACCTGTACTTCCAATCC Protein Sequence ENLYFQS
6xHis	—	6xHis affinity tag	447..465	Rev
DNA Sequence CACCATCATCATCATCAT Protein Sequence HHHHHH

promoter 2

Label	Gene	Product	Position	Strand
T7 promoter	—	—	537..556	Rev
DNA Sequence TAATACGACTCACTATAGG
lacI promoter	lacI	—	864..942	Fwd
DNA Sequence GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	512..537	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC