DIRAS1

38818 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
DIRAS1 (Diras1 Human)
Tags
His Tev
Available Since
March 18, 2013
Availability
Available

Sequence Information

Length
913 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 35..53 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 77..98 Fwd
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
6xHis — 6xHis affinity tag 757..775 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 841..890 Fwd
DNA Sequence
CTAGCATAACCCCTTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG