GPX2

38796 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
GPX2 (GPX2 Human)
Tags
His Tev
Mutation
SeCys40 -> Cys
Available Since
March 18, 2013
Availability
Available

Sequence Information

Length
883 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 41..59 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 83..104 Fwd
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
6xHis — 6xHis affinity tag 727..745 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 811..858 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAACGGGTCTTGAGGGGTTTTTTG