pKM263-ScPPX

38327 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Florian Freimoser
Article
Werner et al Arch Microbiol. 2005 Nov;184(2):129-36.
Insert
PPX1 (PPX1 Budding Yeast)
Tags
GST, His, and TEV
Promoter
T7
Available Since
Aug. 2, 2012
Availability
Available

Sequence Information

Length
834 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 34..52 Fwd
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 709..730 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGGGC
Protein Sequence
ENLYFQG