pTH734-CEN-minHIS3

38227 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Depositor
Tobias von der Haar
Article
Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.
Insert
HIS3 (HIS3 Budding Yeast, Synthetic)
Tags
HA
Mutation
Every codon of the coding sequence has been repla…
Promoter
TDH3
Available Since
Dec. 19, 2012
Availability
Available

Sequence Information

Length
851 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 2
Label Gene Product Position Strand
T3 promoter — — 520..539 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter — — 607..638 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 583..600 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 652..674 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
terminator 1
Label Gene Product Position Strand
ADH2 terminator — — 173..473 Fwd
DNA Sequence
GCGGATCTCTTATGTCTTTACGATTTATAGTTTTCATTATCAAGTATGCCTATATTAGTATATAGCATCTTTAGATGACAGTGTTCGAAGTTTCACGAATAAAAGATAATATTCTACTTTTTGCTCCCACCGCGTTTGCTAGCACGAGTGAACACCATCCCTCGCCTGTGAGTTGTACCCATTCCTCTAAACTGTAGACATGGTAGCTTCAGCAGTGTTCGTTATGTACGGCATCCTCCAACAAACAGTCGGTTATAGTTTGTCCTGCTCCTCTGAATCGTCTCCCTCGATATTTCTCAT