pET21a-MKP7_K62R_153

37795 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
SeWon Suh
Article
Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10
Insert
Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
Tags
His
Mutation
changed Lysnie 62 to Arginine and deleted amino a…
Available Since
Sept. 27, 2012
Availability
Available

Sequence Information

Length
900 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 15..33 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 555..574 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 530..555 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC