pET21a-MKP7_wild_153

37792 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
SeWon Suh
Article
Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10
Insert
Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
Tags
His
Mutation
deleted amino acids 154-665
Available Since
July 13, 2012
Availability
Available

Sequence Information

Length
718 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 24..42 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 555..574 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 530..555 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC