pET16b-Trx CC32/35SS

36946 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Anna Blom
Article
King et al J Immunol. 2012 Apr 15;188(8):4103-12. E
Insert
Thioredoxin 1 (TXN Human)
Tags
His tag
Mutation
both active site cysteines (32,35) mutated to ser…
Promoter
T7
Available Since
May 20, 2013
Availability
Available

Sequence Information

Length
770 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
10xHis — 10xHis affinity tag 34..64 Fwd
DNA Sequence
CATCATCATCATCATCATCATCATCATCAC
Protein Sequence
HHHHHHHHHH
Factor Xa site — Factor Xa recognition and cleavage site 76..88 Fwd
DNA Sequence
ATCGAAGGTCGT
Protein Sequence
IEGR
promoter 1
Label Gene Product Position Strand
tet promoter — — 695..724 Rev
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
terminator 1
Label Gene Product Position Strand
T7 terminator — — 473..521 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG