pET16b-Trx80

36945 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Anna Blom
Article
King et al J Immunol. 2012 Apr 15;188(8):4103-12. E
Insert
Trx80 (truncated Trx) (TXN Human)
Tags
His tag
Mutation
premature stop inserted after K80 in Trx1 gene
Promoter
T7
Available Since
May 20, 2013
Availability
Available

Sequence Information

Length
769 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
10xHis — 10xHis affinity tag 36..66 Fwd
DNA Sequence
CATCATCATCATCATCATCATCATCATCAC
Protein Sequence
HHHHHHHHHH
Factor Xa site — Factor Xa recognition and cleavage site 78..90 Fwd
DNA Sequence
ATCGAAGGTCGT
Protein Sequence
IEGR
promoter 1
Label Gene Product Position Strand
tet promoter — — 697..726 Rev
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
terminator 1
Label Gene Product Position Strand
T7 terminator — — 475..523 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG