pNRB150

36451 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Douglas Bishop
Article
Hong et al J Biol Chem. 2001 Nov 9;276(45):41906-12
Insert
DMC1 (DMC1 Budding Yeast)
Tags
6His
Promoter
T7
Available Since
Aug. 29, 2012
Availability
Available

Sequence Information

Length
919 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
6xHis 6xHis affinity tag 149..167 Fwd
DNA Sequence
CACCATCATCACCATCAT
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter 58..77 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator 77..102 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC