unc5bCD4d3+4-bio-linker-His

36150 Danio rerio Bacterial Expression

Plasmid Information

Purpose
EXPRESs plasmid for Zebrafish Receptors encoding zebrafish unc5b (netrin-1a) with rat CD4d3+4-bio-linker-His tag
Depositor
Gavin Wright
Article
Sollner et al Genome Biol. 2009;10(9):R99. Epub 2009 S
Insert
unc5b (unc5b Zebrafish)
Tags
His tag, biotinylation peptide, and ratCD4d3+4
Mutation
L2V
Promoter
CMV
Available Since
May 9, 2012
Availability
Available

Sequence Information

Length
797 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 340..358 Rev
DNA Sequence
CACCACCATCACCACCAT
Protein Sequence
HHHHHH
polyA_signal 1
Label Gene Product Position Strand
beta-globin poly(A) signal — — 233..289 Fwd
DNA Sequence
TGAGAGACACAAAAAATTCCAACACACTATTGCAATGAAAATAAATTTCCTTTATT