v2rl1_R (TAL 3005)

35997 Danio rerio Bacterial Expression

Plasmid Information

Depositor
Keith Joung
Article
Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.
Insert
TAL array targeting gene- v2rl1 transcript-Q4VDB3_DANRE (v2rl1 Zebrafish)
Tags
3X Flag and WT FOKI
Available Since
March 30, 2012
Availability
Available

Sequence Information

Length
822 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
3xFLAG — three tandem FLAG(R) epitope tags, foll… 79..145 Fwd
DNA Sequence
GACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGATTACAAGGATGACGATGACAAG
Protein Sequence
DYKDHDGDYKDHDIDYKDDDDK
SV40 NLS — nuclear localization signal of SV40 (si… 151..172 Fwd
DNA Sequence
CCCAAGAAGAAGAGGAAGGTG
Protein Sequence
PKKKRKV
promoter 1
Label Gene Product Position Strand
T7 promoter — — 35..54 Fwd
DNA Sequence
TAATACGACTCACTATAGG