pET28a-Vx3(A7)

35526 Synthetic Bacterial Expression

Plasmid Information

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3(A7)

Tags

His6

Mutation

Changed G2 to P

Promoter

T7

Available Since

April 6, 2012

Availability

Available

Sequence Information

Length

685 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 3

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	27..45	Rev
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH
thrombin site	—	thrombin recognition and cleavage site	319..337	Rev
DNA Sequence CTGGTGCCGCGCGGCAGC Protein Sequence LVPRGS
6xHis	—	6xHis affinity tag	346..364	Rev
DNA Sequence CATCATCATCATCATCAC Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	444..463	Rev
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	419..444	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC