pQTEV-EPSTI1

34826 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Konrad Buessow
Article
Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:
Insert
epithelial stromal interaction 1 (EPSTI1 Human)
Tags
His and TEV
Available Since
March 22, 2013
Availability
Available

Sequence Information

Length
876 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
7xHis — 6xHis affinity tag 154..175 Fwd
DNA Sequence
CATCACCATCACCATCACCAT
Protein Sequence
HHHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 199..220 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGGGA
Protein Sequence
ENLYFQG
myr — N-myristoylation signal from Src kinase… 635..653 Fwd
DNA Sequence
GGCAGCAGCAAGAGCAAG
Protein Sequence
GSSKSK
promoter 1
Label Gene Product Position Strand
T5 promoter — — 43..88 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 2
Label Gene Product Position Strand
lac operator — — 63..80 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
lac operator — — 95..112 Fwd
DNA Sequence
TTGTGAGCGGATAACAA