pQTEV-MGC20398

34825 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Konrad Buessow
Article
Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:
Insert
MGC20398 (ZNF830 Human)
Tags
His and TEV
Available Since
Nov. 20, 2012
Availability
Available

Sequence Information

Length
880 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
7xHis — 6xHis affinity tag 165..186 Fwd
DNA Sequence
CATCACCATCACCATCACCAT
Protein Sequence
HHHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 210..231 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGGGA
Protein Sequence
ENLYFQG
promoter 1
Label Gene Product Position Strand
T5 promoter — — 54..99 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 2
Label Gene Product Position Strand
lac operator — — 74..91 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
lac operator — — 106..123 Fwd
DNA Sequence
TTGTGAGCGGATAACAA