pQTEV-MPP6 — PlasmidDB

Plasmid Information

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

membrane protein, palmitoylated 6 (PALS2 Human)

Length

800 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
7xHis	—	6xHis affinity tag	153..174	Fwd
DNA Sequence CATCACCATCACCATCACCAT Protein Sequence HHHHHHH
TEV site	—	tobacco etch virus (TEV) protease recog…	198..219	Fwd
DNA Sequence GAGAATCTTTATTTTCAGGGA Protein Sequence ENLYFQG

promoter 1

Label	Gene	Product	Position	Strand
T5 promoter	—	—	42..87	Fwd
DNA Sequence TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	62..79	Fwd
DNA Sequence TTGTGAGCGGATAACAA
lac operator	—	—	94..111	Fwd
DNA Sequence TTGTGAGCGGATAACAA