GRM3 (3SM9)

32870 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
GRM3 (GRM3 Human)
Tags
His tag with TEV cleavage and Honeybee melittin S…
Available Since
Jan. 13, 2012
Availability
Available

Sequence Information

Length
815 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 158..176 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 197..218 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
sig_peptide 1
Label Gene Product Position Strand
melittin signal sequence — signal sequence from honeybee melittin 83..146 Fwd
DNA Sequence
ATGAAATTCTTAGTCAACGTTGCCCTTGTTTTTATGGTCGTATACATTTCTTACATCTATGCG