LHP398 - Ub-K48R/K63R

32167 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Depositor

Linda Hicke

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Mutation

K48R and K63R

Promoter

CUP1

Available Since

Sept. 27, 2011

Availability

Available

Sequence Information

Length

747 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 1

Label	Gene	Product	Position	Strand
ubiquitin	Homo sapiens UBC	human ubiquitin	2..230	Fwd
DNA Sequence ATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTTGAATCTTCCGATACCATCGACAACGTTAAGTCGAAAATTCAAGACAAGGAAGGCATTCCACCTGATCAACAAAGATTGATCTTTGCCGGTAGACAGCTCGAGGACGGTAGAACGCTGTCTGATTACAACATTCAGAGGGAGTCGACCTTACATCTTGTCTTAAGACTAAGAGGTGGT Protein Sequence MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG

promoter 1

Label	Gene	Product	Position	Strand
TRP1 promoter	S. cerevisiae TRP1	—	622..724	Fwd
DNA Sequence AATTCGGTCGAAAAAAGAAAAGGAGAGGGCCAAGAGGGAGGGCATTGGTGACTATTGAGCACGTGAGTATACGTGATTAAGCACACAAAGGCAGCTTGGAGT

terminator 1

Label	Gene	Product	Position	Strand
CYC1 terminator	S. cerevisiae CYC1	—	342..590	Fwd
DNA Sequence TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC