LHP308 - Ub - wt

32151 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Depositor
Linda Hicke
Article
Terrell et al Mol Cell. 1998 Jan;1(2):193-202.
Insert
synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
Promoter
CUP1
Available Since
Sept. 27, 2011
Availability
Available

Sequence Information

Length
826 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
ubiquitin Homo sapiens UBC human ubiquitin 7..235 Fwd
DNA Sequence
ATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTTGAATCTTCCGATACCATCGACAACGTTAAGTCGAAAATTCAAGACAAGGAAGGCATTCCACCTGATCAACAAAGATTGATCTTTGCCGGTAAGCAGCTCGAGGACGGTAGAACGCTGTCTGATTACAACATTCAGAAGGAGTCGACCTTACATCTTGTCTTAAGACTAAGAGGTGGT
Protein Sequence
MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
terminator 1
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 — 347..595 Fwd
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC