LHP306 - Ub - no Lys

Plasmid Information

Depositor

Linda Hicke

Article

Terrell et al Mol Cell. 1998 Jan;1(2):193-202.

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Mutation

No Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63R

Promoter

CUP1

Available Since

Sept. 27, 2011

Availability

Available

Length

855 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
ubiquitin-K0	—	human ubiquitin variant in which all of…	7..235	Fwd
DNA Sequence ATGCAGATCTTCGTCAGAACGTTAACCGGTAGAACCATAACTCTAGAAGTTGAACCATCCGATACCATCGAAAACGTTAGAGCTAGAATTCAAGACAGAGAAGGCATTCCACCTGATCAACAAAGATTGATCTTTGCCGGTAGACAGCTCGAGGACGGTAGAACGCTGTCTGATTACAACATTCAGAGAGAGTCGACCTTACATCTTGTCTTAAGACTAAGAGGTGGT Protein Sequence MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG

terminator 1

Label	Gene	Product	Position	Strand
CYC1 terminator	S. cerevisiae CYC1	—	347..595	Fwd
DNA Sequence TCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC