MCTS1 — PlasmidDB

Plasmid Information

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

MCTS1 (MCTS1 Human)

Length

764 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	15..33	Rev
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH
6xHis	—	6xHis affinity tag	82..100	Rev
DNA Sequence CACCATCATCACCACCAT Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	714..733	Rev
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	689..714	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC