pQTEV-ASF1A

31591 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Konrad Buessow
Article
Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:
Insert
ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)
Tags
His and TEV
Available Since
Nov. 20, 2012
Availability
Available

Sequence Information

Length
808 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
7xHis — 6xHis affinity tag 165..186 Fwd
DNA Sequence
CATCACCATCACCATCACCAT
Protein Sequence
HHHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 210..231 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGGGA
Protein Sequence
ENLYFQG
promoter 1
Label Gene Product Position Strand
T5 promoter — — 54..99 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 2
Label Gene Product Position Strand
lac operator — — 74..91 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
lac operator — — 106..123 Fwd
DNA Sequence
TTGTGAGCGGATAACAA