pQTEV-MAPRE1

31589 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Konrad Buessow
Article
Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:
Insert
microtubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)
Tags
His and TEV
Available Since
March 22, 2013
Availability
Available

Sequence Information

Length
854 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
7xHis — 6xHis affinity tag 155..176 Fwd
DNA Sequence
CATCACCATCACCATCACCAT
Protein Sequence
HHHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 200..221 Fwd
DNA Sequence
GAGAATCTTTATTTTCAGGGA
Protein Sequence
ENLYFQG
promoter 1
Label Gene Product Position Strand
T5 promoter — — 44..89 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 2
Label Gene Product Position Strand
lac operator — — 64..81 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
lac operator — — 96..113 Fwd
DNA Sequence
TTGTGAGCGGATAACAA