pQStrep2-GADD45G

31584 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Konrad Buessow
Article
Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:
Insert
growth arrest and DNA-damage-inducible, gamma (GADD45G Human)
Tags
His and Strep
Available Since
March 22, 2013
Availability
Available

Sequence Information

Length
860 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 160..178 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
Strep-Tag II — peptide that binds Strep-Tactin(R), an … 667..691 Fwd
DNA Sequence
TGGAGCCACCCGCAGTTCGAAAAA
Protein Sequence
WSHPQFEK
promoter 1
Label Gene Product Position Strand
T5 promoter — — 43..88 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 2
Label Gene Product Position Strand
lac operator — — 63..80 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
lac operator — — 95..112 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 713..808 Fwd
DNA Sequence
GACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAAT