pQStrep2-ARPC3

31580 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Konrad Buessow
Article
Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:
Insert
Actin related protein 2/3 complex, subunit 3 (ARPC3 Human)
Tags
His and StrepII-tag
Available Since
March 25, 2013
Availability
Available

Sequence Information

Length
853 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 161..179 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
Strep-Tag II — peptide that binds Strep-Tactin(R), an … 725..749 Fwd
DNA Sequence
TGGAGCCACCCGCAGTTCGAAAAA
Protein Sequence
WSHPQFEK
promoter 1
Label Gene Product Position Strand
T5 promoter — — 44..89 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 2
Label Gene Product Position Strand
lac operator — — 64..81 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
lac operator — — 96..113 Fwd
DNA Sequence
TTGTGAGCGGATAACAA