pQStrep2-CRYM

31293 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Konrad Buessow
Article
Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:
Insert
crystallin, mu (CRYM Human)
Tags
His and TEV
Available Since
Nov. 20, 2012
Availability
Available

Sequence Information

Length
790 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 171..189 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 674..683 Fwd
DNA Sequence
TAAGGAGGT
promoter 1
Label Gene Product Position Strand
T5 promoter — — 54..99 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 2
Label Gene Product Position Strand
lac operator — — 74..91 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
lac operator — — 106..123 Fwd
DNA Sequence
TTGTGAGCGGATAACAA