pET-HJURP-CBP

30393 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Yawen Bai
Article
Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.
Insert
HJURP CenpA Binding Domain (CENPA Human, Budding Yeast)
Tags
His
Available Since
July 11, 2011
Availability
Available

Sequence Information

Length
932 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 35..53 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
8xHis — 8xHis affinity tag 408..432 Fwd
DNA Sequence
CACCACCACCACCACCACCACCAC
Protein Sequence
HHHHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 508..556 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG