Erk1

29578 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Dustin Maly
Article
Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi
Insert
Erk1 (MAPK3 Human)
Tags
FLAG, HIS6, and TEV
Mutation
K289N mutation present. Starting Met removed from…
Available Since
Sept. 19, 2011
Availability
Available

Sequence Information

Length
951 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 34..52 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 76..97 Fwd
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS