NFATC2IP

28284 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Cheryl Arrowsmith
Insert
NFATC2IP (NFATC2IP Human)
Tags
His tag with TEV cleavage
Mutation
Contains N-terminal ubiquitin-like domain (residu…
Available Since
April 14, 2011
Availability
Available

Sequence Information

Length
891 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 60..78 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 90..111 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
promoter 1
Label Gene Product Position Strand
tet promoter — — 710..739 Rev
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
terminator 1
Label Gene Product Position Strand
T7 terminator — — 488..536 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG