pET-sCS4T

28249 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Yawen Bai
Article
Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.
Insert
single chain Cse4-Scm3-H4 with Thrombin site
Tags
His
Mutation
A thrombin cutting site between Cse4 and Scm3
Available Since
July 12, 2011
Availability
Available

Sequence Information

Length
947 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 35..53 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 293..311 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
8xHis — 8xHis affinity tag 746..770 Fwd
DNA Sequence
CACCACCACCACCACCACCACCAC
Protein Sequence
HHHHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 846..894 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG