pGal4AD-CIBN

28246 Arabidopsis thaliana Yeast Expression

Plasmid Information

Purpose
Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation
Depositor
Chandra Tucker
Article
Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:
Insert
CIB1 (1-171aa)
Tags
Gal4AD
Available Since
March 23, 2011
Availability
Available

Sequence Information

Length
818 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
SV40 NLS nuclear localization signal of SV40 (si… 189..210 Fwd
DNA Sequence
CCAAAAAAGAAGAGAAAGGTC
Protein Sequence
PKKKRKV
GAL4 activation domain Saccharomyces cerevisiae GAL4 (truncated) activation domain of the GAL4 transcrip… 225..567 Fwd
DNA Sequence
GCCAATTTTAATCAAAGTGGGAATATTGCTGATAGCTCATTGTCCTTCACTTTCACTAACAGTAGCAACGGTCCGAACCTCATAACAACTCAAACAAATTCTCAAGCGCTTTCACAACCAATTGCCTCCTCTAACGTTCATGATAACTTCATGAATAATGAAATCACGGCTAGTAAAATTGATGATGGTAATAATTCAAAACCACTGTCACCTGGTTGGACGGACCAAACTGCGTATAACGCGTTTGGAATCACTACAGGGATGTTTAATACCACTACAATGGATGATGTATATAACTATCTATTCGATGATGAAGATACCCCACCAAACCCAAAAAAAGAG
Protein Sequence
ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE
promoter 1
Label Gene Product Position Strand
T7 promoter 572..591 Fwd
DNA Sequence
TAATACGACTCACTATAGG