P100 (3OMC)

28159 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
SND1 (SND1 Human)
Tags
His tag with TEV cleavage
Mutation
Tudor domain (residues 650-910).
Available Since
Feb. 28, 2011
Availability
Available

Sequence Information

Length
782 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 37..55 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site tobacco etch virus (TEV) protease recog… 67..88 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG