UBH14

28158 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
E3 ligase UHRF2 (UHRF2 Human)
Tags
His tag with thrombin cleavage
Mutation
SRA Domain (residues 419-648)
Available Since
Feb. 28, 2011
Availability
Available

Sequence Information

Length
773 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 49..67 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 76..94 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS