pGEX-2T FRB R2109A

26609 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Jie Chen
Article
Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72
Insert
mTOR (MTOR Human)
Tags
GST
Mutation
FRB domain only (aa 2015-2114); R2109A
Available Since
Oct. 28, 2010
Availability
Available

Sequence Information

Length
897 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
FRB — FKBP-rapamycin binding domain of human … 27..306 Fwd
DNA Sequence
ATCCTCTGGCATGAGATGTGGCATGAAGGCCTGGAAGAGGCATCTCGTTTGTACTTTGGGGAAAGGAACGTGAAAGGCATGTTTGAGGTGCTGGAGCCCTTGCATGCTATGATGGAACGGGGCCCCCAGACTCTGAAGGAAACATCCTTTAATCAGGCCTATGGTCGAGATTTAATGGAGGCCCAAGAGTGGTGCAGGAAGTACATGAAATCAGGGAATGTCAAGGACCTCACCCAAGCCTGGGACCTCTATTATCATGTGTTCGCACGAATCTCAAAG
Protein Sequence
ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFARISK
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 622..727 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT