pMal672H

25817 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Cynthia Wolberger
Article
Feeser et al J Mol Biol. 2008 Jul 11. 380(3):520-31.
Insert
Rap1(672-827) (RAP1 Budding Yeast)
Tags
His6 and MBP
Available Since
July 26, 2010
Availability
Available

Sequence Information

Length
865 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
Genenase(TM) I site recognition and cleavage site for Genen… 26..44 Fwd
DNA Sequence
CCGGGTGCGGCACACTAC
Protein Sequence
PGAAHY
TEV site tobacco etch virus (TEV) protease recog… 74..95 Fwd
DNA Sequence
GAAAATTTATATTTTCAAGGT
Protein Sequence
ENLYFQG
thrombin site thrombin recognition and cleavage site 560..578 Fwd
DNA Sequence
TTAGTTCCCCGGGGTTCT
Protein Sequence
LVPRGS
6xHis 6xHis affinity tag 578..596 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
protein_bind 2
Label Gene Product Position Strand
attB1 mutant version of attB 47..72 Fwd
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT
attB2 mutant version of attB 599..624 Rev
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT