ARL5

25525 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
ARL5 (ARL5A Human)
Tags
His
Available Since
Sept. 23, 2010
Availability
Available

Sequence Information

Length
922 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 20..38 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 601..619 Rev
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 628..646 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 726..745 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 701..726 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC