CDY

25507 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
CDY (CDY2B Human)
Tags
His
Mutation
catalytic domain
Available Since
Sept. 10, 2010
Availability
Available

Sequence Information

Length
787 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 469..487 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 496..514 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 370..389 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 389..414 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC