UBH26

25467 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
ubh26.019.099 (UBQLN3 Human)
Tags
His
Mutation
Contains amino acids 19-99
Available Since
Aug. 23, 2010
Availability
Available

Sequence Information

Length
927 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 56..74 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 83..101 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 373..391 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
rep_origin 1
Label Gene Product Position Strand
f1 ori — — 541..901 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTTGATTTA
terminator 1
Label Gene Product Position Strand
T7 terminator — — 457..505 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG