ECT2

25338 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
ECT2:V237-E330 (ECT2 Human)
Tags
His
Mutation
Contains amino acids V237-E330
Available Since
Aug. 17, 2010
Availability
Available

Sequence Information

Length
882 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 35..53 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
6xHis — 6xHis affinity tag 397..415 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 481..529 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG