RALGDS

25331 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
RALGDS:N793-F914 (RALGDS Human)
Tags
His
Mutation
Contains amino acids N793-F914
Available Since
Sept. 23, 2010
Availability
Available

Sequence Information

Length
521 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 460..478 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 490..511 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
promoter 1
Label Gene Product Position Strand
T7 promoter — — 370..389 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 389..414 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC