UBH12 (3BI7)

25219 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
ubh12.414.617 (UHRF1 Human)
Tags
His
Mutation
SRA Domain: Residues 414-617
Available Since
Aug. 12, 2010
Availability
Available

Sequence Information

Length
925 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 660..678 Fwd
DNA Sequence
CACCATCATCACCACCAT
Protein Sequence
HHHHHH
6xHis — 6xHis affinity tag 727..745 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 811..859 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG