LMNB1 (3HN9)

25177 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
LAMINB1_1 (LMNB1 Human)
Tags
His
Mutation
Contains Ig-like domain
Available Since
Aug. 9, 2010
Availability
Available

Sequence Information

Length
916 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 44..62 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 74..95 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
6xHis — 6xHis affinity tag 493..511 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 577..625 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG